"The Separation of Light from Darkness" is the first of the nine paintings in the series on the central ceiling of the Sistine Chapel, depicting in chronological order the creation of the world, the creation of humanity, and humanity's fall. 

The panel depicts God separating the land from the waters. 

The panel shows God creating the sun, moon, and stars. 

Genesis chapter two explains how God created Eve from Adam's rib to provide him companionship. They were later expelled from the Garden of Eden for disobeying God's will, a pivotal scene for religious believers. 

The panel depicts Adam and Eve being tempted and then expelled from the Garden of Eden. 

The flood contains over sixty figures, some clinging to a tilted boat in the middle ground. Survivors of the flood desperately seek refuge on a small piece of land still available to them. One person climbs a tree, futilely trying to escape the rising waters, while another holds the lifeless body of a drowned child, attempting a failed rescue mission. All await the inevitable destruction. In the background, the ark floats on calm waters, symbolizing hope amidst the frantic human tragedy scene.

This painting allows viewers to see up close the desperation of those who are about to perish in the flood. 

Noah and his family sacrifice to God in order to safely escape from the great flood. 

The panel depicts the scene of Noah getting drunk.